1. Use a clean glass slide.
2. Take a loop of the culture.
3. Place the live microorganism on the glass slide.
4. The slice is air dried then passed over a Bunsen burner about three times.
5. The heat causes the microorganism to adhere to the glass slide. This is known as fixing the microorganism to the glass slide.
1884
Hans Christian Gram
Developed the Gram stain used to stain and identify bacteria.
Franz Ziehl and Friedrick Nielsen
Developed the Ziehl-Nielsen acid-fast stain used to stain bacteria.
Year
Scientists
Contribution
not yet complete table is ,issing
GRAM STAINING TECHNIQUE
1. Prepare the specimen using the heat fixation process (Smear)
2. Place a drop of crystal violet stain on the specimen.
3. Apply iodine on the specimen. The iodine helps the crystal violet stains adhere to the specimen. Iodine is a mordant, which is a chemical that fixes the stain to the specimen.
4. Rinse in water
5. Wash the specimen with an ethanol or alcohol-acetone decolorizing solution.
6. Rinse the specimen with water to remove the dye.
